Prostatic Acid Phosphatase/ACPP in Human Prostate. Prostatic Acid Phosphatase/ACPP was detected in immersion fixed paraffin-embedded sections of human prostate using Human Prostatic Acid Phosphatase/ACPP Antigen Affinity-purified Polyclonal Antibody at 3 ug/ml overnight at 4°C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells.
Detection of Human Prostatic Acid Phosphatase/ACPP by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF Membrane was probed with 1 ug/ml of Human Prostatic Acid Phosphatase/ACPP Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Prostatic Acid Phosphatase/ACPP at approximately 50 -55 kD (as indicated). This experiment was conducted under reducing conditions.