Formalin-fixed and paraffin-embedded human breast carcinoma reacted with ACTN4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of ACTN4 Antibody in T47D, A2058, A375, HepG2 cell line and mouse bladder tissue lysates (35 ug/lane). ACTN4 (arrow) was detected using the purified antibody.
Flow cytometric of MDA-231 cells using ACTN4 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.