ACTN4

Gene Symbol ACTN4
Entrez Gene 81
Alt Symbol ACTININ-4, FSGS, FSGS1
Species Human
Gene Type protein-coding
Description actinin, alpha 4
Other Description F-actin cross-linking protein|actinin alpha4 isoform|alpha-actinin-4|focal segmental glomerulosclerosis 1|non-muscle alpha-actinin 4
Swissprots O76048 D6PXK4 A4K467 O43707
Accessions EAW56809 EAW56810 EAW56811 O43707 AA478431 AA847056 AK299955 BAG61782 AK304554 BAG65349 AK309361 AK309363 AK309364 AU118403 BC005033 AAH05033 BC015620 AAH15620 BM841805 BP332596 BX647098 D89980 BAA24447 DQ431186 ABD96103 DQ893439 ABM84365 DQ896777 ABM87776 GU987085 ADG03678 U48734 AAC17470 XM_005259281 XP_005259338 XM_005259282 XP_005259339 XM_006723406 XP_006723469 NM_004924 NP_004915
Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778). {ECO:0000250|UniProtKB:P57780, ECO:0000269|PubMed:15772161, ECO:0000269|PubMed:22351778, ECO:0000305|PubMed:9508771}.
Subcellular Location Nucleus {ECO:0000269|PubMed:22351778, ECO:0000269|PubMed:9508771}. Cytoplasm {ECO:0000269|PubMed:22351778, ECO:0000269|PubMed:9508771}. Cell junction {ECO:0000250|UniProtKB:P57780}. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines (breast cancer cell line MCF-7, oral floor cancer IMC- 2, and bladder cancer KU-7). {ECO:0000269|PubMed:17289661, ECO:0000269|PubMed:9508771}.
Tissue Specificity Widely expressed. {ECO:0000269|PubMed:9508771}.
Top Pathways Systemic lupus erythematosus, Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Leukocyte transendothelial migration, Amoebiasis

α-actinin (B-12) - sc-166524 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
α-actinin (B-12): sc-166524. Western blot analysis of α-actinin expression in L8 (A) and HeLa (B) whole cell lysates.

α-actinin (N-19) - sc-7453 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
α-actinin (N-19): sc-7453. Western blot analysis of α-actinin expression in A-673 (A), HeLa (B), L8 (C) and NIH/3T3 (D) whole cell lysates and rat skeletal muscle tissue extract (E).

α-actinin (H-300) - sc-15335 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
α-actinin (H-300): sc-15335. Immunofluorescence staining of methanol-fixed A-673 cells showing cytoplasmic staining.

α-actinin (C-20)-R - sc-7454-R from Santa Cruz Biotechnology

Prices $61.00
Sizes 100 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
α-actinin (C-20): sc-7454. Western blot analysis of α-actinin expression in C2C12 (A), Sol8 (B), NIH/3T3 (C), MCP-5 (D), 3T3-L1 (E) and c4 (F) whole cell lysates.

α-actinin-4 (A-8) - sc-393495 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
α-actinin-4 (A-8): sc-393495. Western blot analysis of α-actinin-4 expression in HeLa (A), A-673 (B), MCF7 (C), SJRH30 (D), K-562 (E) and A-431 (F) whole cell lysates.

α-actinin-4 (B-11) - sc-390180 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
α-actinin-4 (B-11): sc-390180. Western blot analysis of α-actinin-4 expression in HeLa whole cell lysate.

α-actinin-4 (D-3) - sc-398088 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
α-actinin-4 (D-3): sc-398088. Western blot analysis of α-actinin-4 expression in NIH/3T3 (A), A-673 (B), HeLa (C), MCF7 (D), SJRH30 (E) and K-562 (F) whole cell lysates.

α-actinin-4 (G-4) - sc-390205 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
α-actinin-4 (G-4): sc-390205. Western blot analysis of α-actinin-4 expression in MCF7 (A), SJRH30 (B), K-562 (C), A-431 (D) and 3T3-L1 (E) whole cell lysates.

α-actinin-4 (H-51) - sc-135346 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
α-actinin-4 (H-51): sc-135346. Western blot analysis of α-actinin-4 expression in non-transfected: sc-117752 (A) and human α-actinin-4 transfected: sc-176210 (B) 293T whole cell lysates.

α-actinin-4 (K-14) - sc-49332 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
α-actinin-4 (K-14): sc-49332. Western blot analysis of α-actinin-4 expression in NIH/3T3 whole cell lysate.

α-actinin-4 (N-17) - sc-49333 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
α-actinin-4 (N-17): sc-49333. Western blot analysis of α-actinin-4 expression in HeLa whole cell lysate.

α-actinin-4 (LW-M23) - sc-134236 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
α-actinin-4 (LW-M23): sc-134236. Western blot analysis of α-actinin-4 expression in non-transfected 293T: sc-117752 (A), human α-actinin-4 transfected 293T: sc-176191 (B) and HeLa (C) whole cell lysates.

Anti-alpha Actinin 4 (phospho S159) antibody - ab117411 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-alpha Actinin 4 antibody - ab59468 from Abcam

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-alpha Actinin 4 antibody (ab59468) at 1/1000 dilution + Whole cell lysates prepared from mouse embryonic fibroblasts at 10 µgSecondaryHRP conjugated goat polyclonal to rabbit IgG at 1/3000 dilutionPerformed under reducing conditions.

Anti-alpha Actinin 4 antibody - ab96686 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-alpha Actinin 4 antibody (ab96686) at 1/1000 dilutionLane 1 : A431 whole cell lysateLane 2 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-alpha Actinin 4 antibody - ab119945 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-alpha Actinin 4 antibody (ab119945) at 1/1000 dilutionLane 1 : A549 cell lysateLane 2 : HeLa cell lysateLane 3 : MCF7 cell lysateLane 4 : A431 cell lysateLane 5 : Human fetal muscle lysate

Anti-alpha Actinin 4 antibody - ab76665 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-alpha Actinin 4 antibody (ab76665) at 1/50 dilution + HeLa cell lysate at 35 µg

Anti-alpha Actinin 4 antibody [4D10] - ab117445 from Abcam

Prices $384.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4D10
Anti-alpha Actinin 4 antibody [4D10] (ab117445) + PC12 lysate

Anti-alpha Actinin 4 antibody [7H6] - ab32816 from Abcam

Prices $392.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 7H6
ab32816 (4 µg/ml) staining alpha actinin in human lung, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of both cytoplasm and nuclei of the bronchial epithelium and resident macrophage cells.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-alpha Actinin 4 antibody [EPR2533(2)] - ab108198 from Abcam

Prices $397.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2533(2)
All lanes : Anti-alpha Actinin 4 antibody [EPR2533(2)] (ab108198) at 1/1000 dilutionLane 1 : Fetal skeletal muscle lysateLane 2 : A431 cell lysateLane 3 : MCF-7 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.

Anti-alpha Actinin 4 antibody [EPR2533(2)] (Alexa Fluor® 488) - ab198608 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2533(2)
ab198608 staining alpha Actinin 4 in MCF7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198608 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in MCF7 cells fixed with 4% formaldehyde (10 min).

Anti-alpha Actinin 4 antibody [EPR2533(2)] (Alexa Fluor® 647) - ab198610 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2533(2)
ab198610 staining alpha Actinin 4 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198610 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in MCF7 cells fixed with 100% methanol (5 min).

Anti-alpha Actinin 4 antibody [EPR2533(2)] (HRP) - ab199072 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2533(2)
All lanes : Anti-alpha Actinin 4 antibody [EPR2533(2)] (HRP) (ab199072) at 1/5000 dilutionLane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)Lysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-alpha Actinin 4 antibody [EPR4410] - ab108201 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4410
All lanes : Anti-alpha Actinin 4 antibody [EPR4410] (ab108201) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF-7 cell lysateLane 3 : C2C12 cell lysateLane 4 : A673 cell lysateLane 5 : L6 cell lysateLysates/proteins at 10 µg per lane.

Anti-alpha Actinin 4 antibody - C-terminal - ab137564 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-alpha Actinin 4 antibody - C-terminal (ab137564) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
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