Detection of Human JARID1D/SMCY by Western Blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer.
Antibody: Affinity purified rabbit anti-JARID1D/SMCY antibody A301-751A (lot A301-751A-2) used for WB at 0.1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human JARID1D/SMCY by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer.
Antibody: Affinity purified rabbit anti-JARID1D/SMCY antibody A301-751A (lot A301-751A-2) used for IP at 6 µg per reaction. For blotting immunoprecipitated JARID1D/SMCY, A301-751A was used at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.