Western blot analysis of extracts from F9 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® DNMT3L siRNA I (Mouse Specific) (+), or SignalSilence® DNMT3L siRNA II (Mouse Specific) #12308 (+), using DNMT3L Antibody (Mouse Specific) #12309 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The DNMT3L Antibody (Mouse Specific) confirms silencing of DNMT3L expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length mouse DNMT3L (mDNMT3L-Myc; +), and F9 cells using DNMT3L Antibody (Mouse Specific).
Immunoprecipitation of DNMT3L from F9 cell extracts, using Normal Rabbit IgG #2729 (lane 2) or DNMT3L Antibody (Mouse Specific) (lane 3). Lane 1 is 10% input. Western blot analysis was performed using DNMT3L Antibody (Mouse Specific).