Western blot analysis of extracts from neonatal mouse brain and fetal rat brain using Phospho-Doublecortin (Ser334) Antibody. The phospho-specificity of the antibody was verified by blocking with a phospho- or non-phosphopeptide and by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after western transfer.
Confocal immunofluorescent analysis of postnatal day 14 rat brain, untreated (left) or λ-phosphatase treated (right), using Phospho-Doublecortin (Ser334) Antibody (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).