DCX

Gene Symbol DCX
Entrez Gene 1641
Alt Symbol DBCN, DC, LISX, SCLH, XLIS
Species Human
Gene Type protein-coding
Description doublecortin
Other Description doublecortex|doublin|lis-X|lissencephalin-X|neuronal migration protein doublecortin
Swissprots Q5JYZ5 D3DUY9 O43911 D3DUY8 A6NFY6 O43602 A9Z1V8 D3DUZ0
Accessions CAA06617 EAX02642 EAX02643 EAX02644 EAX02645 EAX02646 EAX02647 EAX02648 EAX02649 O43602 AF034634 AAC52037 AF040254 AAC31797 AF040255 AAC31696 AJ003112 CAA05867 AK002120 AK290455 BAF83144 AK297297 BAG59765 AK299822 BAG61691 AU120758 AW163329 BC027925 AAH27925 DA497601 DC375659 HQ447825 ADQ32309 XM_011530878 XP_011529180 XM_011530879 XP_011529181 XM_011530880 XP_011529182 NM_000555 NP_000546 NM_001195553 NP_001182482 NM_178151 NP_835364 NM_178152 NP_835365 NM_178153 NP_835366
Function Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration. {ECO:0000269|PubMed:22359282}.
Subcellular Location Cytoplasm. Cell projection {ECO:0000250}. Note=Localizes at neurite tips. {ECO:0000250}.
Tissue Specificity Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.

Phospho-Doublecortin (Ser334) Antibody - 3453 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from neonatal mouse brain and fetal rat brain using Phospho-Doublecortin (Ser334) Antibody. The phospho-specificity of the antibody was verified by blocking with a phospho- or non-phosphopeptide and by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after western transfer.

Phospho-Doublecortin (Ser297) Antibody - 4605 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from fetal rat and fetal human brain using Phospho-Doublecortin (Ser297) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after Western transfer.

Doublecortin Antibody - 4604 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from fetal rat brain and rat brain, using Doublecortin Antibody (upper) or α-Actinin (D6F6) XP ® Rabbit mAb #6487 (lower).

Doublecortin (D7E4) Rabbit mAb - 3973 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone D7E4
Western blot analysis of extracts from mouse and rat brain using Doublecortin (D7E4) Rabbit mAb.

Doublecortin (E-5) - sc-390645 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Doublecortin (E-5): sc-390645. Western blot analysis of Doublecortin expression in non-transfected 293T: sc-117752 (A), human Doublecortin transfected 293T: sc-114231 (B) and IMR-32 (C) whole cell lysates.

Doublecortin (E-6) - sc-271390 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Doublecortin (E-6): sc-271390. Western blot analysis of Doublecortin expression in non-transfected: sc-117752 (A) and human Doublecortin transfected: sc-114231 (B) 293T whole cell lysates.

Doublecortin (C-18) - sc-8066 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Doublecortin (C-18): sc-8066. Immunofluorescence staining of Doublecortin (red) and Neu N (green) in dentate gyrus of seven day post-ischemic rat brain perfused with 4% paraformaldehyde at low magnification (A,C,E) and thin optical sections (~1 µm) under high magnification (B,D,F) demonstrating that doublecortin identifies newly formed neurons. Kindly provided by Dr. Dan Small and Dr. Robert Monette from the Institute for Biological Sciences, National Research Council, Ottawa, Canada.

Doublecortin (H-280) - sc-28939 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Doublecortin (H-280): sc-28939. Western blot analysis of Doublecortin expression in non-transfected 293T: sc-117752 (A), human Doublecortin transfected 293T: sc-114231 (B) and SK-N-SH (C) whole cell lysates.

Doublecortin (N-19) - sc-8067 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Doublecortin (C-18): sc-8066. Immunofluorescence staining of Doublecortin (red) and Neu N (green) in dentate gyrus of seven day post-ischemic rat brain perfused with 4% paraformaldehyde at low magnification (A,C,E) and thin optical sections (~1 µm) under high magnification (B,D,F) demonstrating that doublecortin identifies newly formed neurons. Kindly provided by Dr. Dan Small and Dr. Robert Monette from the Institute for Biological Sciences, National Research Council, Ottawa, Canada.

Anti-Doublecortin (phospho S28) antibody - Neuronal Marker - ab23544 from Abcam

Prices $380.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Doublecortin (phospho S28) antibody - Neuronal Marker (ab23544) at 1 µg/mlLane 1 : Mouse Adult Brain Whole Tissue LysateLane 2 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188)Lane 3 : Mouse Adult Brain Whole Tissue Lysate with Mouse Doublecortin (phospho S28) peptide (ab31511) at 1 µg/mlLane 4 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) with Mouse Doublecortin (phospho S28) peptide (ab31511) at 1 µg/mlLane 5 : Mouse Adult Brain Whole Tissue Lysate with Mouse Doublecortin peptide (ab31512) at 1 µg/mlLane 6 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) with Mouse Doublecortin peptide (ab31512) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Doublecortin antibody - ab18723 from Abcam

Prices $401.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab18723 staining Doublecortin in mouse embryonic 15 day brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.5% Triton X-100 in PBS, blocked with 10% serum for 1 hour at 25°C and antigen retrieval was by heat mediation in citrate buffer, pH 6. The sample was incubated with primary antibody (1/500 in PBS + 0.1% Triton X-100 + 1% serum) for 16 hours at 25°C. An Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal (1/700) was used as the secondary antibody.See Abreview

Anti-Doublecortin antibody - ab54739 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-Doublecortin antibody (ab54739) at 5 µg/ml + immunogen at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Anti-Doublecortin antibody - ab75863 from Abcam

Prices $378.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Doublecortin antibody (ab75863) at 1/50 dilution + K562 cell line lysate at 35 µg

Anti-Doublecortin antibody - ab153668 from Abcam

Prices $370.00
Sizes 500 µl
Host Chicken
Clonality Polyclonal

Anti-Doublecortin antibody - ab61121 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Doublecortin antibody (ab61121) at 1/500 dilutionLane 1 : Extracts from RAW264.7cellsLane 2 : Extracts from RAW264.7cells with immunising Doublecortin peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-Doublecortin antibody [2G5] - ab135349 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2G5
Flow cytometric analysis of SK-N-SH cells labelling Doublecortin with ab135349 at 1/200 dilution (green) or negative control (red).

Anti-Doublecortin antibody [4A3] - ab119033 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4A3
All lanes : Anti-Doublecortin antibody [4A3] (ab119033) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Doublecortin cDNALysates/proteins at 5 µg per lane.

Anti-Doublecortin antibody [EPR10935(B)] - ab167400 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10935(B)
All lanes : Anti-Doublecortin antibody [EPR10935(B)] (ab167400) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : C6 lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-Doublecortin antibody [EPR10936] - ab172631 from Abcam

Host Rabbit
Clonality Monoclonal
Clone EPR10936
Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal brain lysate or (Lane 2) 1XPBS (negative control) using ab172631 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

Anti-Doublecortin antibody - Neuronal Marker - ab77450 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Doublecortin antibody - Neuronal Marker (ab77450) at 1 µg/ml + Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) at 10 µgSecondaryRabbit IgG secondary antibody (ab28446) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Doublecortin antibody - Neuronal Marker - ab23542 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Doublecortin antibody - Neuronal Marker (ab23542) at 1 µg/mlLane 1 : Mouse brain lysateLane 2 : Mouse day 0 brain lysateLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to rabbit IgG (Alexa fluor 680) at 1/10000 dilution

Anti-Doublecortin antibody - Neuronal Marker - ab28941 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab28941 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28941, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

Anti-DCX / Doublecortin Antibody (clone 2G5) IHC-plus™ - LS-B8946 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2G5
Anti-DCX / Doublecortin antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-DCX / Doublecortin Antibody (aa284-297) - LS-C155045 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
DCX antibody (0.5 ug/ml) staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-DCX / Doublecortin Antibody (aa151-161) - LS-C139686 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
DCX antibody (0.01 ug/ml) staining of Mouse fetal Brain lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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