Western blot analysis of extracts from HeLa cells, untreated or treated with Calyculin A #9902, using Phospho-DRP1 (Ser616) Antibody.
Western blot analysis of extracts from HeLa cells, untreated or nocodazole-treated for the indicated times, using Phospho-DRP1 (Ser616) Antibody.
Immunoprecipitation of Phospho-DRP1 (Ser616) from HeLa cell extracts, untreated or nocodazole-treated, using Phospho-DRP1 (Ser616) Antibody followed by western blot using the same antibody. Lanes 1 & 2 are 5% input.
Confocal immunofluorescent analysis of NCI-H1299 cells, untreated (left) or λ-phosphatase-treated (right), using Phospho-DRP1 (Ser616) Antibody (green). Actin filaments have been labeled with DY-554 phallodin (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).
Flow cytometric analysis of Jurkat cells using Phospho-DRP1 (Ser616) Antibody versus propidium iodide (DNA content).