DNM1L

Gene Symbol DNM1L
Entrez Gene 10059
Alt Symbol DLP1, DRP1, DVLP, DYMPLE, EMPF, HDYNIV
Species Human
Gene Type protein-coding
Description dynamin 1-like
Other Description Dnm1p/Vps1p-like protein|dynamin family member proline-rich carboxyl-terminal domain less|dynamin-1-like protein|dynamin-like protein 4|dynamin-like protein IV|dynamin-related protein 1
Swissprots Q59GN9 A8K4X9 Q7L6B3 B4DGC9 O60709 Q8TBT7 Q9Y5J2 O14541 O00429 J3KPI2 Q9BWM1 B4DSU8
Accessions EAW88519 EAW88520 EAW88521 EAW88522 EAW88523 EAW88524 EAW88525 EAW88526 EAW88527 O00429 AA236613 AB006965 BAA22193 AB209070 BAD92307 AF000430 AAC23724 AF061795 AAC35283 AF151685 AAD39541 AK123906 AK291094 BAF83783 AK293290 BAG56814 AK294533 BAG57740 AK298568 BAG60761 AK299926 BAG61760 AK302565 BAG63828 AK311606 BC024590 AAH24590 BC107763 AAI07764 BF725436 DC377686 DQ891544 ABM82470 DQ894732 ABM85658 XM_005253282 XP_005253339 XM_005253283 XP_005253340 XM_011520543 XP_011518845 XM_011520544 XP_011518846 NM_001278463 NP_001265392 NM_001278464 NP_001265393 NM_001278465 NP_001265394 NM_001278466 NP_001265395 NM_005690 NP_005681 NM_012062 NP_036192 NM_012063 NP_036193
Function Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane- associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage. Required for normal brain development, including that of cerebellum. Facilitates developmentally regulated apoptosis during neural tube formation. Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues. Also required for mitochondrial fission during mitosis. Required for formation of endocytic vesicles. Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulate
Subcellular Location Cytoplasm, cytosol. Golgi apparatus. Endomembrane system; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein. Peroxisome. Membrane, clathrin-coated pit {ECO:0000250}. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250}. Note=Mainly cytosolic. Translocated to the mitochondrial membrane through O- GlcNAcylation and interaction with FIS1. Recruited to the mitochondrial outer membrane by interaction with MIEF1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Localizes to mitochondria following necrosis induction. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex. Binds to phospholipid membranes.
Tissue Specificity Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons. {ECO:0000269|PubMed:10749171, ECO:0000269|PubMed:9422767, ECO:0000269|PubMed:9570752, ECO:0000269|PubMed:9731200, ECO:0000269|PubMed:9786947}.
Top Pathways TNF signaling pathway

Phospho-DRP1 (Ser616) (D9A1) Rabbit mAb - 4494 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9A1
Western blot analysis of extracts from HeLa cells, untreated (-) or treated (+) with nocodazole (100 ng/ml, 17 hr), using Phospho-DRP1 (Ser616) (D9A1) Rabbit mAb (upper) and DRP1 (D6C7) Rabbit mAb #8570 (lower).

Phospho-DRP1 (Ser637) (D3A4) Rabbit mAb - 6319 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3A4
Western blot analysis of extracts from PC-12 cells, untreated or treated with Forskolin #3828 (20 μM, 1 hr), using Phospho-DRP1 (Ser637) (D3A4) Rabbit mAb.

Phospho-DRP1 (Ser616) Antibody - 3455 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, untreated or treated with Calyculin A #9902, using Phospho-DRP1 (Ser616) Antibody.

Phospho-DRP1 (Ser637) Antibody - 4867 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from PC-12 cells, untreated or treated with 20uM Forskolin #3828 for 1 hour, using Phospho-DRP1 (Ser637) Antibody.

DRP1 (D6C7) Rabbit mAb - 8570 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6C7
Western blot analysis of extracts from various cell lines using DRP1 (D6C7) Rabbit mAb.

DRP1 (D8H5) Rabbit mAb - 5391 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D8H5
Western blot analysis of extracts from various cell lines using DRP1 (D8H5) Rabbit mAb.

DRP1 (4E11B11) Mouse mAb - 14647 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 4E11B11
Western blot analysis of extracts from various cell lines using DRP1 (4E11B11) Mouse mAb.

DRP1 (C-5) - sc-271583 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DRP1 (C-5): sc-271583. Western blot analysis of DRP1 expression in HeLa whole cell lysate.

DRP1 (H-16) - sc-21804 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
DRP1 (H-16): sc-21804. Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic staining of glandular cells.

DRP1 (H-300) - sc-32898 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
DRP1 (H-300): sc-32898. Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

DRP1 (6Z-82) - sc-101270 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
DRP1 (6Z-82): sc-101270. Western blot analysis of DRP1 expression in non-transfected 293T: sc-117752 (A), human DRP1 transfected 293T: sc-113653 (B) and HeLa (C) whole cell lysates.

Anti-DRP1 (phospho S637) antibody - ab193216 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DRP1 (phospho S637) antibody (ab193216) at 1/500 dilutionLane 1 : Jurkat cell extractLane 2 : K562 cell extractLane 3 : K562 cell extract with antigen-specific peptide

Anti-DRP1 antibody - ab56788 from Abcam

Prices $403.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-DRP1 antibody - ab154879 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of DRP1 staining in mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab154879, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-DRP1 antibody - ab118926 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-DRP1 antibody (ab118926) at 1 µg/ml + Hela cell lysate at 10 µg

Anti-DRP1 antibody - ab123599 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DRP1 antibody (ab123599) at 0.4 µg/mlLane 1 : Whole cell lysate from HeLa cells at 50 µgLane 2 : Whole cell lysate from HeLa cells at 15 µgLane 3 : Whole cell lysate from 293T cells at 50 µgLane 4 : Whole cell lysate from Jurkat cells at 50 µgLane 5 : Whole cell lysate from NIH 3T3 cells at 50 µgdeveloped using the ECL techniqueExposure time : 3 minutes

Anti-DRP1 antibody - ab54038 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DRP1 antibody (ab54038) at 2 µg/mlLane 1 : Drp1-mutLane 2 : OPA1-nullLane 3 : Double mutLane 4 : Mfn2-nullLane 5 : Mfn1-nullLane 6 : Wildtype.

Anti-DRP1 antibody [2E7] - ab140494 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2E7
Overlay histogram showing HEK293 cells stained with ab140494 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140494, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-DRP1 antibody [3F4] - ab150336 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F4
All lanes : Anti-DRP1 antibody [3F4] (ab150336) at 1/4000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY controlLane 2 : HEK293T cells transfected with pCMV6-ENTRY DNM1L Lysates/proteins at 5 µg per lane.

Anti-DRP1 antibody [4F6] - ab156951 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4F6
All lanes : Anti-DRP1 antibody [4F6] (ab156951) at 1/200 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with pCMV6-ENTRY DRP1 cDNALysates/proteins at 5 µg per lane.

Anti-DRP1 antibody - C-terminal - ab180769 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling DRP1 with ab180769 at 1/100. Magnification: 200x.

Anti-DRP1 antibody - C-terminal - ab196948 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DRP1 antibody - C-terminal (ab196948) at 1/500 dilutionLane 1 : HuvEc cell extract with synthesized peptideLane 2 : HuvEc cell extractLane 3 : HepG2 cell extractLane 4 : Colo cell extractLane 5 : 293 cell extractLane 6 : Jurkat cell extract

Anti-DNM1L / DRP1 Antibody (clone 3F4) - LS-C338047 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F4
IHC of paraffin-embedded Human bladder tissue using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Anti-DNM1L / DRP1 Antibody (clone 3F3) - LS-C174691 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F3
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-DNM1L mouse monoclonal antibody.

Anti-DNM1L / DRP1 Antibody (clone 2E7) - LS-C174692 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2E7
IHC of paraffin-embedded Human pancreas tissue using anti-DNM1L mouse monoclonal antibody.
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