Western Blot analysis of extracts from HeLa, SK-N-MC, and A-673 cells using E2F-1 Antibody.
Western blot analysis of extracts from HeLa cells, untransfected (-) or transfected with E2F1 siRNA (+) to silence E2F1 protein expression, using E2F-1 Antibody.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 Raji cells and either 5 μl of E2F-1 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using using SimpleChIP ® Human Timeless Intron 1 Primers #7001, human DHFR promoter primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.