Western blot analysis of mouse Brain Tissue Lysate using PA5-19478, PSD95 (phospho T19) primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19478 staining of Mouse Forebrain Tissue Lysate at a dilution of 1 ug/ml (Lane 2). PA5-19478 staining of Rat Brain Tissue Lysate at a dilution of 1 ug/ml (lane 3). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
Immunohistochemical (formalin-fixed, paraffin-embedded) staining of Human Hippocampus tissue using PA5-19478, anti-PSD95 antibody. Primary antibody was used at a concentration of 1 ug/ml and exposed for 15 mins at room temp. The sample was pretreated using heat mediated antigen retrieval with Sodium Citrate Buffer (pH6 / 20mins). The detection method was a HRP conjugated polymer, DAB chromogen and the sample was counterstained with haematoxylin and mounted with DPX.