The ability of Human EGF Neutralizing (D8A1) Rabbit mAb to inhibit hEGF-induced MCF 10A cell proliferation was assessed. MCF 10A cells were treated with increasing concentrations of antibody in the presence of hEGF #8916 (2 ng/ml). After 24 hr, cells were labeled with BrdU for 4 hr and BrdU incorporation was determined using BrdU Cell Proliferation Assay Kit #6813.
The proliferation of MCF 10A cells treated with increasing concentrations of hEGF #8916 was assessed. After 24 hr, cells were labeled with BrdU for 4 hr and BrdU incorporation was determined using BrdU Cell Proliferation Assay Kit #6813.