EGF

Gene Symbol EGF
Entrez Gene 1950
Alt Symbol HOMG4, URG
Species Human
Gene Type protein-coding
Description epidermal growth factor
Other Description beta-urogastrone|pro-epidermal growth factor
Swissprots Q52LZ6 P01133 B4DRK7 E9PBF0 E7EVD2
Accessions AAR84237 CCQ43157 EAX06257 P01133 AI628974 AK299306 BAG61319 AK307734 AY548762 AAS83395 BC093731 AAH93731 BC113461 AAI13462 BC143357 CA437074 CU446298 DB227969 DB349864 J02548 AAA72173 JQ346088 AFA26280 X04571 CAA28240 XM_005262796 XP_005262853 XM_005262797 XP_005262854 XM_005262798 XP_005262855 XM_005262800 XP_005262857 XM_005262801 XP_005262858 XM_005262802 XP_005262859 XM_006714124 XP_006714187 XM_011531707 XP_011530009 XM_011531708 XP_011530010 XM_011531709 XP_011530011 XR_427532 XR_938699 NM_001178130 NP_001171601 NM_001178131 NP_001171602 NM_001963 NP_001954
Function EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. {ECO:0000269|PubMed:17671655}.
Subcellular Location Membrane; Single-pass type I membrane protein.
Tissue Specificity Expressed in kidney, salivary gland, cerebrum and prostate. {ECO:0000269|PubMed:17671655}.
Top Pathways Endocytosis, Rap1 signaling pathway, Hepatitis C, PI3K-Akt signaling pathway, Choline metabolism in cancer

Human EGF Neutralizing (D8A1) Rabbit mAb - 12157 from Cell Signaling Technology

Prices $338.00
Sizes 100 µg
Host Rabbit
Clonality Monoclonal
Clone D8A1
The ability of Human EGF Neutralizing (D8A1) Rabbit mAb to inhibit hEGF-induced MCF 10A cell proliferation was assessed. MCF 10A cells were treated with increasing concentrations of antibody in the presence of hEGF #8916 (2 ng/ml). After 24 hr, cells were labeled with BrdU for 4 hr and BrdU incorporation was determined using BrdU Cell Proliferation Assay Kit #6813.

EGF (D-5) - sc-374255 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
EGF (D-5): sc-374255. Western blot analysis of human recombinant EGF fusion protein.

EGF (F-9) - sc-166779 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
EGF (F-9): sc-166779. Western blot analysis of EGF expression in human platelet extract.

EGF (H-3) - sc-271090 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
EGF (H-3): sc-271090. Western blot analysis of human recombinant EGF (sc-4552).

EGF (C-20) - sc-1341 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
EGF (C-20): sc-1341. Western blot analysis of human recombinant EGF fusion protein.

EGF (R-20) - sc-1343 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
EGF (R-20): sc-1343. Western blot analysis of human recombinant EGF fusion protein.

EGF (Z-12) - sc-275 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
EGF (Z-12): sc-275. Western blot analysis of human recombinant EGF.

Anti-EGF antibody - ab9695 from Abcam

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-EGF antibody (ab9695) at 0.2 µg/mlLane 1 : Human EGF full length protein (ab55483) at 0.1 µgLane 2 : Human EGF full length protein (ab55483) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 1 minute

Anti-EGF antibody - ab171327 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of formalin fixed and paraffin embedded Human brain tissue labeling EGF using ab171327 at a 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

Anti-EGF antibody - ab94898 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-EGF antibody - ab157757 from Abcam

Host Sheep
Clonality Polyclonal

Anti-EGF antibody [144-8] - ab54422 from Abcam

Prices $359.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal
Clone 144-8

Anti-EGF antibody [1B11-3E12] - ab40644 from Abcam

Host Mouse
Clonality Monoclonal
Clone 1B11-3E12

Anti-EGF antibody [3A8] (Biotin) - ab155576 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3A8
Sandwich ELISA analysis of Human EGF using ab155576.

Anti-EGF antibody (Biotin) - ab84263 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-EGF antibody [EGF-10] - ab10409 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone EGF-10
ab10409 staining EGF in human normal skin tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with formaldehyde. Samples were blocked with 10% serum for 30 minutes at 21°C followed by incubation with the primary antibody at a 1/500 dilution for 30 minutes at 21°C. An undiluted HRP-conjugated goat polyclonal was used as the secondary antibody.See Abreview

Anti-EGF antibody [EGF-2] - ab8282 from Abcam

Prices $378.00
Sizes 500 µg
Host Mouse
Clonality Monoclonal
Clone EGF-2
ICC/IF image of ab8282 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8282, 5µg/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-EGF antibody [KT2] (HRP) - ab106489 from Abcam

Prices $368.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone KT2
Sandwich ELISA using a mouse monoclonal antibody to EGF coated plate and ab106489 at 1/1000 dilution.

Anti-EGF antibody [S-147] - ab18632 from Abcam

Prices $376.00
Sizes 250 µg
Host Mouse
Clonality Monoclonal
Clone S-147
ICC/IF image of ab18632 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18632, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-EGF antibody [S-177] - ab18635 from Abcam

Prices $376.00
Sizes 250 µg
Host Mouse
Clonality Monoclonal
Clone S-177
Standard Curve for EGF (Analyte: ab51082) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [S-177] to EGF (ab18635) at 5ug/ml and Detector Antibody Rabbit polyclonal to EGF (ab9695) at 0.5ug/ml

Anti-EGF antibody [S-21] - ab18630 from Abcam

Prices $370.00
Sizes 250 µg
Host Mouse
Clonality Monoclonal
Clone S-21

Anti-EGF Antibody - LS-C23133 from LifeSpan Bioscience

Prices $495.00
Sizes 500 µg
Host Mouse
Clonality Monoclonal

Anti-EGF Antibody - LS-C23134 from LifeSpan Bioscience

Prices $495.00
Sizes 500 µg
Host Mouse
Clonality Monoclonal

Anti-EGF Antibody - LS-C23135 from LifeSpan Bioscience

Prices $495.00
Sizes 500 µg
Host Mouse
Clonality Monoclonal

Anti-EGF Antibody - LS-C23138 from LifeSpan Bioscience

Prices $495.00
Sizes 500 µg
Host Mouse
Clonality Monoclonal
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