Immunofluorescent analysis of ZFAT showing staining in the nucleus of SKNSH cells. SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a ZFAT polyclonal antibody (Product # PA5-32037) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 ?m.
Western blot analysis of ZFAT using 30µg of A) Jurkat and B) Raji lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a ZFAT polyclonal antibody (Product # PA5-32037) at a dilution of 1:1000.
Immunohistochemical analysis of ZFAT in paraffin-embedded HBL435 xenograft using a ZFAT polyclonal antibody (Product # PA5-32037) at a dilution of 1:500.