Pol II siRNA (h): sc-36290. Western blot analysis of Pol II expression in non-transfected control (A) and Pol II siRNA transfected (B) HeLa cells. Blot probed with Pol II (A-10): sc-17798. α-actinin (H-2): sc-17829 used as specificity and loading control.
p-Pol II (8A7): sc-13583. Western blot analysis of Pol II phosphorylation in untreated (A), heat shocked (C) and calyculin-treated (E) HeLa cultures. Total Pol II levels were determined in the same respective extracts by using Pol II (A-10): sc-17798 (B,D,F).
Pol II (A-10): sc-17798. Western blot analysis of Pol II isoform expression in A-673 (A) and NIH/3T3 (B) nuclear extracts and AT-3 (C) whole cell lysate.