Western blot analysis of Pol II expression in A-431 (A,D), AT-3 (B,E) and A-673 (C,F) nuclear extracts. Antibodies tested include Pol II (C-21): sc-900 (A-C) and Pol II (N-20): sc-899 (D-F).
ChIP analysis of recruitment of basal transcription factors Pol II and TBP to the IL-2Rα (CD25) promoter in vivo. Antibodies tested include Pol II (C-21): sc-900 and TFIID (TBP) (SI-1): sc-273. CD3ε and Oct-2 promoter regions were employed as positive and negative controls, respectively. DNA was isolated from Rag
-/- or WT thymocytes. Data kindly provided by J. Imbert and reproduced from Yeh, J.H., et al. 2002. Nucleic Acids Res. 30: 1944-1951, with permission from Oxford University Press.