Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ASPA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent of ASPA Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot of ASPA (arrow) using rabbit polyclonal ASPA Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ASPA gene.
Western blot of ASPA Antibody in MCF7 cell line lysates (35 ug/lane). ASPA (arrow) was detected using the purified antibody.
Flow cytometric of MDA-MB468 cells using ASPA Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.