Anti-Elg1 Antibody - Western Blot. Western blot of Affinity Purified anti-Elg1 antibody shows detection of a band ~120 kD corresponding to human Elg1 (arrowhead) in various cell lysates. Lanes contain ~5 ug of HeLa nuclear extract (1), HeLa (2), A431 (3), Jurkat (4) and HEK293 (5) whole cell lysates. After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500. The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2 m exposure time. The expected molecular weight of Elg1 is 120kD according to Kanellis P et al. 2003, although the predicted molecular weight is 207 kD. The 50kD bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins. Both the 120 kD and 50 kD bands are not observed when antibody is pre-incubated with peptide (data not shown).