Anti-ATP5J Antibody (aa27-56)
| Name | Anti-ATP5J Antibody (aa27-56) |
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-C156660 |
| Prices | $295.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC-P ICC/IF WB |
| Species Reactivities | Human, Mouse |
| Antigen | ATP5J antibody was raised against kLH-conjugated synthetic peptide from internal region of human ATP5J. |
| Purity/Format | Immunoaffinity purified |
| Blocking Peptide | ATP5H Antibody Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | ATP5J |
| Supplier Page | Shop |
Product images
ATP5J Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Fluorescent confocal image of U251 cell stained with ATP5J Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ATP5J primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ATP5J immunoreactivity is localized to Mitochondria significantly.
Western blot of lysate from rat heart tissue lysate, using ATP5J Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
ATP5J Antibody western blot of mouse stomach tissue lysates (35 ug/lane). The ATP5J antibody detected the ATP5J protein (arrow).