Detection of Human ATRIP by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (100 ug for WB; 400 ug for IP) from HeLa (A & B) and 293-T (A) cells. Antibodies: Affinity purified rabbit anti-ATRIP antibody used at the indicated concentrations for WB and 3 ug/0.4 mg lysate for IP. ATRIP was also Immunoprecipitated using a control antibody generated to recombinant human ATRIP. Immunoprecipitated ATRIP was detected using the antibody to the recombinant protein. Detection: Chemiluminescence with exposure times of 5 minutes (A) and 45 seconds (B).