ATRIP

Gene Symbol	ATRIP
Entrez Gene	84126
Alt Symbol	-
Species	Human
Gene Type	protein-coding
Description	ATR interacting protein
Other Description	ATM and Rad3-related-interacting protein\|ATR-interacting protein
Swissprots	Q96CL3 Q8WXE1 Q8NHQ2 Q9HA30 Q8WUG7 A8K714 A8K6A3 B5MEB7 Q69YK9 B2RCE7 B4DU92
Accessions	EAW64875 EAW64876 EAW64877 EAW64878 EAW64879 EAW64880 EAW64881 EAW64882 Q8WXE1 AF451323 AAL38042 AK022405 BAB14029 AK291568 BAF84257 AK291829 BAF84518 AK300548 BAG62254 AK315075 BAG37544 AL832917 CAH10621 BC014153 AAH14153 BC020563 AAH20563 BC030597 AAH30597 CB960784 DC402185 DQ892238 ABM83164 DQ895438 ABM86364 NM_001271022 NP_001257951 NM_001271023 NP_001257952 NM_032166 NP_115542 NM_130384 NP_569055
Function	Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein. {ECO:0000269\|PubMed:12791985}.
Subcellular Location	Nucleus {ECO:0000269\|PubMed:11721054, ECO:0000269\|PubMed:18283122}. Note=Redistributes to discrete nuclear foci upon DNA damage.
Tissue Specificity	Ubiquitous. {ECO:0000269\|PubMed:11721054}.
Top Pathways	Fanconi anemia pathway

Phospho-ATRIP (Ser224) Antibody - 5161 from Cell Signaling Technology

Prices	$287.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from 293T cells, untreated or treated with λ and calf intestinal phosphatases, using Phospho-ATRIP (Ser224) Antibody (upper) or ATRIP Antibody #2737 (lower).

ATRIP Antibody - 2737 from Cell Signaling Technology

Prices	$99.00, $246.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from Jurkat and HEL cells using ATRIP Antibody.

ATRIP (F-7) - sc-365383 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

ATRIP (F-7): sc-365383. Western blot analysis of ATRIP expression in non-transfected: sc-117752 (A) and human ATRIP transfected: sc-170063 (B) 293T whole cell lysates.

Anti-ATRIP (phospho S224) antibody - ab138385 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ATRIP (phospho S224) antibody (ab138385) at 1/500 dilution + 293 cell lysate - UV treated (15 min) at 30 µg

Anti-ATRIP (phospho S239) antibody - ab183323 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-ATRIP (phospho S239) antibody (ab183323) at 1/500 dilutionLane 1 : Mouse testis lysateLane 2 : Mouse testis lysate incubated in lambda-phosphatase (800 units/1mg protein for 30 min).

Anti-ATRIP (phospho S68) antibody - ab182626 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-ATRIP (phospho S68) antibody (ab182626) at 1/500 dilutionLane 1 : Extracts from 3T3 cells with Antigen-specific peptideLane 2 : Extracts from 3T3 cellsdeveloped using the ECL technique

Anti-ATRIP (phospho S68) antibody - ab192192 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-ATRIP (phospho S68) antibody (ab192192) at 1/500 dilutionLane 1 : NIH3T3 whole cell lysate with antigen-specific peptideLane 2 : NIH3T3 whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-ATRIP antibody - ab59346 from Abcam

Prices	$388.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

ab59346, at a 1/50 dilution, staining human ATRIP in lung carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence (left image) and presence of the immunizing peptide (right image).

Anti-ATRIP antibody - ab17962 from Abcam

Prices	$378.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Detection of Human ATRIP using ab17962 by Western Blot (2 x left images) and Immunoprecipitation (right image).Samples: Whole cell lysate (100 µg for WB; 400 µg for IP) from HeLa (A & B) and 293-T (A) cells. Antibodies: Affinity purified rabbit anti-ATRIP antibody (ab17962) used at 1/500 and 1/15000 dilution for WB and 3 µg/0.4 mg lysate for IP. ATRIP was also immunoprecipitated using a control antibody generated to recombinant human ATRIP:Right image - lane 1 ab17962; lane 2 control antibody; lane 3 normal IgG.Immunoprecipitated ATRIP was detected using the antibody to the recombinant protein. Detection: Chemiluminescence with exposure times of 5 minutes (2 x left) and 45 seconds (right image).

Anti-ATRIP antibody - ab56956 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Western blot against tagged recombinant protein immunogen using ab56956 ATRIP antibody at 1ug/ml.

Anti-ATRIP antibody - ab90614 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of Human ATRIP in formalin-fixed paraffin-embedded human prostate carcinoma tissue using ab90614 at a dilution of 1/250

Anti-ATRIP antibody [5E7] - ab128529 from Abcam

Prices	$384.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	5E7

All lanes : Anti-ATRIP antibody [5E7] (ab128529) at 1/500 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with pCMV6-ENTRY ATRIP cDNALysates/proteins at 5 µg per lane.

Anti-ATRIP antibody [EPR8072(2)] - ab175221 from Abcam

Prices	$381.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR8072(2)

Immunofluorescent staining of MCF-7 cells labeling ATRIP with ab175221 at 1/50 dilution.

Anti-ATRIP antibody - Aminoterminal end - ab137517 from Abcam

Prices	$384.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ATRIP antibody - Aminoterminal end (ab137517) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

Anti-ATRIP Antibody (clone 5E7) - LS-C173897 from LifeSpan Bioscience

Prices	$350.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	5E7

IHC of paraffin-embedded Human pancreas tissue using anti-ATRIP mouse monoclonal antibody.

Anti-ATRIP Antibody (Asp64) - LS-C176386 from LifeSpan Bioscience

Prices	$345.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

IHC of ATRIP (D64) pAb in paraffin-embedded human lung colon carcinoma tissue.

Anti-ATRIP Antibody (aa190-239) - LS-C291246 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ATRIP Antibody (phospho-Ser224) - LS-C291547 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ATRIP Antibody (phospho-Ser239) - LS-C319719 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ATRIP Antibody (aa100-150) - LS-C286679 from LifeSpan Bioscience

Prices	$415.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human ATRIP by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ATRIP used at a dilution of 1:250.

Anti-ATRIP Antibody (clone 1G6) - LS-C173892 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	1G6

Western blot of extracts (35 ug) from 9 different cell lines by using g anti-ATRIP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Anti-ATRIP Antibody (aa500-550) - LS-C287027 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human ATRIP by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (100 ug for WB; 400 ug for IP) from HeLa (A & B) and 293-T (A) cells. Antibodies: Affinity purified rabbit anti-ATRIP antibody used at the indicated concentrations for WB and 3 ug/0.4 mg lysate for IP. ATRIP was also Immunoprecipitated using a control antibody generated to recombinant human ATRIP. Immunoprecipitated ATRIP was detected using the antibody to the recombinant protein. Detection: Chemiluminescence with exposure times of 5 minutes (A) and 45 seconds (B).

Anti-ATRIP Antibody (aa100-150) - LS-C287538 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ATRIP Antibody (aa741-791) - LS-C290598 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human ATRIP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ATRIP antibody used for IP at 6 ug/mg lysate. ATRIP was also immunoprecipitated by rabbit anti-ATRIP antibody BL14237. For blotting immunoprecipitated ATRIP, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-ATRIP Antibody - LS-C346249 from LifeSpan Bioscience

Prices	$255.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Immunofluorescence blot of A549 cell using ATRIP antibody.

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