Anti-BLIMP1 / PRDM1 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10615 concentration 5 ug/ml.
Anti-PRDM1/BLIMP1 Antibody - Western Blot. Western blots using affinity purified anti-PRDM1/BLIMP1 antibody show detection of overexpressed PRDM1/BLIMP1 in whole transfected Raji cell lysate (lane 2) at ~88kD. Lane 1 shows mock transfection in whole Raji cell lysate. Detection of endogenous PRDM1/BLIMP1 (lane 4) is illustrated in human plasma cell nuclear extract, but not in Raji whole cell nuclear extract (lane 3). The identity of the lower dark band at ~50-60kD is unknown. Primary antibody was used at a 1:1000 dilution in 5% PBS-Tween. Personal communication, Hyesuk Yoon and Jerry Boss, Emory University, Atlanta, GA.