Western blot analysis of extracts from SNB19 cells, untreated (-) or EphrinA1-F c ligand-treated (100 ng/ml, 5 min; +), using Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb (upper) or EphA2 (D4A2) XP ® Rabbit mAb #6997 (lower).
Immunoprecipitation of phospho-EphA2 (Tyr588) from EphrinA1-F c ligand-treated (100 ng/ml, 5 min) SNB19 cell extracts, using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb.