Detection of Human BIRC6 by Western Blot and Immunoprecipitation. Samples: A) Whole cell lysate (WCL) and S100 extract (S100) from HeLa cells. B) Whole cell lysate (10 mg) from HeLa cells. Antibody: Affinity purified rabbit anti-BIRC6 antibody used at 0.1 ug/ml for WB (A) and at 2 ug/mg lysate for IP (B). Detection: A) Chemiluminescence with an exposure time of 10 minutes. B) Immunoprecipitate was resolved by SDS-PAGE and stained with Coomassie Blue. The band that is BIRC6 was confirmed by mass spectrometry.