Sp3 (D-20): sc-644. Western blot analysis of Sp3 expression in KNRK nuclear extract.
Sp3 (D-20): sc-644. Nuclear immunofluorescence staining of methanol-fixed HeLa cells.
Sp3 siRNA (h): sc-29490. Western blot analysis of Sp3 expression in non-transfected control (A) and Sp3 siRNA transfected (B) HeLa cells. Blot probed with Sp3 (D-20): sc-644. α-actinin (H-2): sc-17829 used as specificity and loading control.
Sp3 (D-20): sc-644. ChIP analysis of Sp3 recruitment to genomic amplicons. Four (1-4) different human genomic Sp-1 and two control amplicons (5,6) were analyzed by quantitative PCR (primer sequences available in on-line supplemental data). Data generated in collaboration with Drs. N. Trinklein and R. Myers, Stanford University (ENCODE Project).
Sp3 (D-20): sc-644. Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.
Sp3 (D-20): sc-644. Western blot analysis of Sp3 expression in non-transfected: sc-117752 (A) and mouse Sp3 transfected: sc-127572 (B) 293T whole cell lysates and HeLa nuclear extract (C).
Sp3 (D-20)-G: sc-644-G. Western blot analysis of Sp3 expression in non-transfected: sc-117752 (A) and mouse Sp3 transfected: sc-127572 (B) 293T whole cell lysates.