Western blot (WB) analysis of p-GRB10 (Y67) pAb at 1:500 dilution Lane1:HEK293T cell lysate treated with insulin(100nM,5mins) Lane2:Mouse spleen tissue lysate Lane3:PC12 cell lysate treated with insulin(100nM,5mins)
Immunohistochemistry (IHC) analyzes of p-GRB10 (Y67) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.