Phospho-p44/42 MAPK (Erk1) (Tyr204)/(Erk2) (Tyr187) (D1H6G) Mouse mAb

• Supplier: Cell Signaling Technology
• Catalog: 5726
• Prices: $287.00
• Sizes: 100 µl (10 western blots)
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: D1H6G
• Applications: WB ICC/IF FC
• Species Reactivities: Human, Mouse, Rat, Monkey, Chicken, Drosophila, Xenopus, Zebrafish, Bovine, C. elegans
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr187 of human Erk2 protein.
• Description: Mouse Monoclonal
• Gene: MAPK3

Product images

Western blot analysis of extracts from various cell lines, serum-starved overnight and untreated (-) or treated with U0126 #9903, hEGF #8916, or TPA #4174 as indicated, using Phospho-p44/42 MAPK (Erk1) (Tyr204)/(Erk2) (Tyr187) (D1H6G) Mouse mAb (upper) or p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb #4696 (lower).

Confocal immunofluorescent analysis of HeLa cells, treated with PDBu (100 nM, 15 min; left) or U0126 #9903 (10 μM, 2 hr; right), using Phospho-p44/42 MAPK (Erk1) (Tyr204)/(Erk2) (Tyr187) (D1H6G) Mouse mAb (green) and β-Actin (13E5) Rabbit mAb #4970 (red).

Flow cytometric analysis of Jurkat cells, treated with U0126 #9903 (10 μM, 2 hr) (blue) or TPA #4174 (200 nM, 15 min) (green), using Phospho-p44/42 MAPK (Erk1) (Tyr204)/(Erk2) (Tyr187) (D1H6G) Mouse mAb. Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4408 was used as a secondary antibody.