MAPK3

Gene Symbol	MAPK3
Entrez Gene	5595
Alt Symbol	ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK
Species	Human
Gene Type	protein-coding
Description	mitogen-activated protein kinase 3
Other Description	MAP kinase isoform p44\|MAPK 1\|extracellular signal-regulated kinase 1\|extracellular signal-related kinase 1\|insulin-stimulated MAP2 kinase\|microtubule-associated protein 2 kinase
Swissprots	Q8NHX1 P27361 A8CZ58 B0LPG3
Accessions	ABY87542 AGC09597 CBK51145 EAW79912 EAW79913 EAW79914 EAW79915 EAW79916 EAW79917 P27361 AK091009 BAG52261 AK307995 AY033607 AAK52329 AY033608 AAK52330 BC000205 AAH00205 BC013992 AAH13992 BX537897 CAD97888 DA351318 DQ399291 ABD60302 DQ893440 ABM84366 DQ896778 ABM87777 KJ534878 AHW56518 M84490 AAA36142 X60188 CAA42744 Z11696 CAA77754 XR_243293 NM_001040056 NP_001035145 NM_001109891 NP_001103361 NM_002746 NP_002737
Function	Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organel
Subcellular Location	Cytoplasm. Nucleus. Note=Autophosphorylation at Thr-207 promotes nuclear localization.
Top Pathways	TGF-beta signaling pathway, Adherens junction, Axon guidance, VEGF signaling pathway, Focal adhesion

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb (HRP Conjugate) - 8544 from Cell Signaling Technology

Prices	$329.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D13.14.4E

Western blot analysis of extracts from either Jurkat cells, treated with U0126 #9903 (10 µM, 1 hr) or TPA #4174 (200 nM, 10 min), or MCF7 cells, treated with λ phosphatase or TPA, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb (HRP Conjugate).

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 4344 from Cell Signaling Technology

Prices	$329.00
Sizes	100 µl (50 tests)
Host	Rabbit
Clonality	Monoclonal
Clone	D13.14.4E

Flow cytometric analysis of Jurkat cells, U0126-treated (blue) or PMA-treated (green), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate).

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb (Biotinylated) - 4094 from Cell Signaling Technology

Prices	$329.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D13.14.4E

Western blot analysis of p44/42 MAPK (Erk1/2) Control Cell Extracts #9194, treated with either U0126 #9903 or TPA #4174, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Biotinylated) #4094.

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 13214 from Cell Signaling Technology

Prices	$299.00
Sizes	100 µl (50 tests)
Host	Rabbit
Clonality	Monoclonal
Clone	197G2

Flow cytometric analysis of Jurkat cells treated with U0126 #9903 (blue) or TPA #4174 (green) using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (Alexa Fluor ® 488 Conjugate).

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (HRP Conjugate) - 14474 from Cell Signaling Technology

Prices	$299.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	197G2

Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or treated with TPA #4174 (200 nM, 10 min; +), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (HRP Conjugate).

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 13148 from Cell Signaling Technology

Prices	$299.00
Sizes	100 µl (50 tests)
Host	Rabbit
Clonality	Monoclonal
Clone	197G2

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (Alexa Fluor ® 647 Conjugate) - 4375 from Cell Signaling Technology

Prices	$299.00
Sizes	100 µl (50 tests)
Host	Mouse
Clonality	Monoclonal
Clone	E10

Flow cytometric analysis of Jurkat cells, UO126-treated (blue) or PMA-treated (green), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (Alexa Fluor ® 647 conjugate) compared to a nonspecific control antibody (red).

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (PE Conjugate) - 14095 from Cell Signaling Technology

Prices	$299.00
Sizes	100 µl (50 tests)
Host	Rabbit
Clonality	Monoclonal
Clone	197G2

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (Pacific Blue™ Conjugate) - 14196 from Cell Signaling Technology

Prices	$299.00
Sizes	100 µl (50 tests)
Host	Rabbit
Clonality	Monoclonal
Clone	197G2

Flow cytometric analysis of Jurkat cells, treated with U0126 #9903 (blue) or PMA-stimulated (green), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (Pacific Blue™ Conjugate).

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (Alexa Fluor ® 488 Conjugate) - 4374 from Cell Signaling Technology

Prices	$299.00
Sizes	100 µl (50 tests)
Host	Mouse
Clonality	Monoclonal
Clone	E10

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (Biotinylated) - 14227 from Cell Signaling Technology

Prices	$299.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	197G2

Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or treated with TPA #4174 (200 nM for 10 min, +), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (Biotinylated).

p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 4780 from Cell Signaling Technology

Prices	$299.00
Sizes	100 µl (50 tests)
Host	Rabbit
Clonality	Monoclonal
Clone	137F5

Flow cytometric analysis of Jurkat cells, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Alexa Fluor ® 488 Conjugate) (green) compared to XP ® Rabbit (DA1E) mAb IgG Isotype Control (Alexa Fluor ® 488 Conjugate) #2975 (blue).

p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (PE Conjugate) - 8867 from Cell Signaling Technology

Prices	$299.00
Sizes	100 µl (50 tests)
Host	Rabbit
Clonality	Monoclonal
Clone	137F5

Flow cytometric analysis of Jurkat cells using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (PE Conjugate) (green) compared to concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control (PE Conjugate) #5742 (red).

p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (HRP Conjugate) - 4348 from Cell Signaling Technology

Prices	$299.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	137F5

Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (HRP Conjugate).

p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Biotinylated) - 5013 from Cell Signaling Technology

Prices	$299.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	137F5

Western blot analysis of extracts from various cell lines using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Biotinylated).

p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 5376 from Cell Signaling Technology

Prices	$299.00
Sizes	100 µl (50 tests)
Host	Rabbit
Clonality	Monoclonal
Clone	137F5

Flow cytometric analysis of Jurkat cells using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Alexa Fluor ® 647 Conjugate) (green) compared to Rabbit (DA1E) mAb IgG XP ® Isotype Control (Alexa Fluor ® 647 Conjugate) #2985 (blue).

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb (Pacific Blue™ Conjugate) - 12638 from Cell Signaling Technology

Host	Rabbit
Clonality	Monoclonal
Clone	D13.14.4E

Flow cytometric analysis of Jurkat cells treated with U0126 #9903 (blue) or TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (green) using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb (Pacific Blue™ Conjugate).

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 4284 from Cell Signaling Technology

Host	Rabbit
Clonality	Monoclonal
Clone	D13.14.4E

Confocal immunofluorescent analysis of MCF7 cells, insulin-treated (100 nM, 10 minutes; left) or treated with U0126 #9903 (10 uM, 2 hours; right), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb (PE Conjugate) - 5682 from Cell Signaling Technology

Host	Rabbit
Clonality	Monoclonal
Clone	D13.14.4E

p44/42 MAPK (Erk1/2) (3A7) Mouse mAb (Alexa Fluor® 647 Conjugate) - 4379 from Cell Signaling Technology

Host	Mouse
Clonality	Monoclonal
Clone	3A7

Flow cytometric analysis of Jurkat cells, U0126-treated (left) or PMA-treated (right), using p44/42 MAPK (3A7) Mouse mAb (Alexa Fluor® 647 Conjugate) and Phospho-p44/42 MAPK (T202/Y204) (E10) Mouse mAb (Alexa Fluor® 488 Conjugate) #4374.

p44/42 MAPK (Erk1/2) (3A7) Mouse mAb (Alexa Fluor® 488 Conjugate) - 4378 from Cell Signaling Technology

Host	Mouse
Clonality	Monoclonal
Clone	3A7

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb - 4370 from Cell Signaling Technology

Prices	$119.00, $318.00, $750.00
Sizes	20 µl (2 western blots), 200 µl (40 western blots), 600 µl (120 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D13.14.4E

Western blot analysis of extracts from COS cells, untreated or treated with either U0126 #9903 (10 µM for 1h) or TPA #4174 (200 nM for 10 m), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb #4370 and p44/42 MAPK (Erk1/2) (3A7) Mouse mAb #9107.

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb - 9106 from Cell Signaling Technology

Prices	$287.00, $678.00
Sizes	200 µl (40 western blots), 600 µl (120 western blots)
Host	Mouse
Clonality	Monoclonal
Clone	E10

Specificity and sensitivity of the Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb: This antibody reacts specifically with as little as 50 pg of phosphorylated MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated MAP kinase by Western blotting.

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody - 9101 from Cell Signaling Technology

Prices	$287.00, $678.00
Sizes	200 µl (20 western blots), 600 µl (60 western blots)
Host	Rabbit
Clonality	Polyclonal

Specificity and sensitivity of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody. The antibody reacts specifically with as little as 0.25 ng of phosphorylated p42 MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated p42 MAP kinase.

Phospho-p44/42 MAPK (Erk1) (Tyr204)/(Erk2) (Tyr187) (D1H6G) Mouse mAb - 5726 from Cell Signaling Technology

Prices	$287.00
Sizes	100 µl (10 western blots)
Host	Mouse
Clonality	Monoclonal
Clone	D1H6G

Western blot analysis of extracts from various cell lines, serum-starved overnight and untreated (-) or treated with U0126 #9903, hEGF #8916, or TPA #4174 as indicated, using Phospho-p44/42 MAPK (Erk1) (Tyr204)/(Erk2) (Tyr187) (D1H6G) Mouse mAb (upper) or p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb #4696 (lower).

Search more