Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 4377
• Prices: $109.00, $307.00
• Sizes: 20 µl (2 western blots), 200 µl (20 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: 197G2
• Applications: WB ICC/IF FC
• Species Reactivities: Human, Mouse, Rat, Monkey, Mink, Drosophila, Zebrafish, Pig
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase.
• Description: Rabbit Monoclonal
• Gene: MAPK3

Product images

Western blot analysis of purified MAPK phospho-proteins or extracts from NIH/3T3 cells treated with UV light and PDGF, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (upper), Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 (middle), and Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb #4671 (lower).

Confocal immunofluorescent analysis of HT-1080 cells, starved overnight then treated with U0126 #9903 (10 μM, 2 hr; left) or PDBu (Phorbol 12,13-Dibutyrate) #12808 (100 nM, 15 min; right) using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb #4377 (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of U0126-inhibited (blue) or PMA-stimulated (green) Jurkat cells, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb compared to a nonspecific negative control antibody (red).