p44/42 MAPK (Erk1/2) Antibody

• Supplier: Cell Signaling Technology
• Catalog: 9102
• Prices: $246.00, $596.00
• Sizes: 200 µl (20 western blots), 600 µl (60 western blots)
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB IP IHC-P ICC/IF FC
• Species Reactivities: Human, Mouse, Rat, Hamster, Monkey, Mink, Zebrafish, Bovine, Pig, Yeast
• Antigen: Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence in the C-terminus of rat p44 MAP Kinase
• Description: Rabbit Polyclonal
• Gene: MAPK3

Product images

Western blot analysis of extracts from HeLa cells transfected with 100 nM control siRNA #6201 (-) or p44 MAPK (Erk1) siRNA (+), using p44/42 MAPK (Erk1/2) Antibody #9102 and GCK Antibody #3782. The Erk1/2 antibody confirms silencing of Erk1 expression, and GCK Antibody is used to control for loading and specificity of p44 MAPK (Erk1) siRNA.

Western blot analysis of extracts from serum-induced PC12 cells, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101 (upper) or control p44/42 MAPK (Erk1/2) Antibody (lower).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using p44/42 MAPK (Erk1/2) Antibody.

Confocal immunofluorescent analysis of HT1080 cells, starved overnight then treated with U0126 #9903 (10 uM, 2 h; left) or PDBu (Phorbol 12,13-Dibutyrate) #12808 (100 nM, 15 m; right) using p44/42 MAPK (Erk1/2) Antibody #9102 (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow cytometric analysis of Jurkat cells, using p44/42 MAPK (Erk1/2) Antibody (blue) compared to a nonspecific negative control antibody (red).