Formalin-fixed, paraffin-embedded tumor/normal adjacent tissue cores from a human colontissue microarray stained for TUCAN/CARD8 expression at 1:2000 using LS-C148305. A and B are tumor tissue cores. A1 and B1 are the matched normal adjacent cores from A and B, respectively. Hematoxylin-Eos in counterstain.
Formalin-fixed, paraffin-embedded tissue human colon microarray stained for TUCAN/CARD8 expression at 1:2000 using LS-C148305. A-A2, successively higher magnifications of the microarray. A, low magnification over view. A1, differential TUCAN/CARD8 staining between tissue cores is observed. A2, Areas of intense TUCAN/CARD8 staining in a colon carcinoma tissue core. Hematoxylin Eosin counterstain.
Western blot of TUCAN/CARD8 in tumor cell lines using LS-C148305 at 1:2000. Cell lysates were normalized for total protein content.