Detection of Mouse DBC1/p30 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3 , Renca, and TCMK-1 cells. Antibodies: Affinity purified goat anti-DBC1/p30 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human DBC1/p30 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. Antibodies: Affinity purified goat anti-DBC1/p30 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.