Detection of Human DBC1/p30 DBC by Immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DBC1/p30 DBC used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Detection of Human DBC1/p30 DBC by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DBC1/p30 DBC antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. DBC1/p30 DBC was also immunoprecipitated by a previous lot (-1) of this antibody. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).
Detection of Mouse DBC1 by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, Renca, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-DBC1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.