Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection of Human TRIP12 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (0.5, 1.5 and 5 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-TRIP12 antibody used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 second (B).