Western blot analysis of extracts from HeLa and H-4-II-E cells serum-starved overnight and TPA-stimulated for 4 hours, using c-Fos (9F6) Rabbit mAb.
Confocal immunofluorescent analysis of HeLa cells serum-starved (left) or treated with TPA (#9905) for 4 hours (right) and labeled with c-Fos (9F6) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
Flow cytometric analysis of Hela cells, untreated (blue) or TPA treated (green), using c-Fos (9F6) Rabbit mAb compared to a nonspecific negative control antibody (red).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 PC-12 cells starved overnight and treated with Human β-Nerve Growth Factor (h-βNGF) #5221 (50ng/ml) for 2h, and either 10 μl of c-Fos (9F6) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Rat CCRN4L Promoter Primers #7983, rat DCLK1 promoter primers, and SimpleChIP ® Rat GAPDH Promoter Primers #7964. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.