FOS

Gene Symbol FOS
Entrez Gene 2353
Alt Symbol AP-1, C-FOS, p55
Species Human
Gene Type protein-coding
Description FBJ murine osteosarcoma viral oncogene homolog
Other Description FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)|G0/G1 switch regulatory protein 7|activator protein 1|cellular oncogene c-fos|proto-oncogene c-Fos
Swissprots B4DQ65 P01100 P18849 A8K4E2
Accessions AAA52471 AAC98315 AAO21129 BAA87920 BAA87921 CAA24756 CBX47570 EAW81227 EAW81228 EAW81229 P01100 AK097379 BAG53458 AK290907 BAF83596 AK291326 BAF84015 AK298659 BAG60827 BC004490 AAH04490 BX647104 CR541785 CAG46584 CR542267 CAG47063 DQ893444 ABM84370 DQ896750 ABM87749 S65138 AAB20306 NM_005252 NP_005243
Function Nuclear phosphoprotein which forms a tight but non- covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD- binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum. {ECO:0000269|PubMed:16055710, ECO:0000269|PubMed:17160021, ECO:0000269|PubMed:22105363, ECO:0000269|PubMed:7588633, ECO:0000269|PubMed:9732876}.
Subcellular Location Nucleus. Endoplasmic reticulum. Cytoplasm, cytosol. Note=In quiescent cells, present in very small amounts in the cytosol. Following induction of cell growth, first localizes to the endoplasmic reticulum and only later to the nucleus. Localization at the endoplasmic reticulum requires dephosphorylation at Tyr-10 and Tyr-30.
Top Pathways Toll-like receptor signaling pathway, cAMP signaling pathway, Circadian entrainment, TNF signaling pathway, Prolactin signaling pathway

Phospho-c-Fos (Ser32) (D82C12) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 8709 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D82C12
Flow cytometric analysis of HeLa cells, untreated (blue) or treated with TPA #4174 (green), using Phospho-c-Fos (Ser32) (D82C12) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate).

Phospho-c-Fos (Ser32) (D82C12) XP ® Rabbit mAb (PE Conjugate) - 11919 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D82C12
Flow cytometric analysis of HeLa cells, untreated (blue) or treated with TPA #4174 (green), using Phospho-c-Fos (Ser32) (D82C12) XP® Rabbit mAb (PE Conjugate).

Phospho-c-Fos (Ser32) (D82C12) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 8677 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D82C12
Flow cytometric analysis of HeLa cells, untreated (blue) or treated with TPA #4174 (green), using Phospho-c-Fos (Ser32) (D82C12) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate).

Phospho-c-Fos (Ser32) (D82C12) XP ® Rabbit mAb - 5348 from Cell Signaling Technology

Prices $119.00, $318.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D82C12
Western blot analysis of extracts from HeLa cells, serum-starved overnight and then either untreated or stimulated for 4 hours with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174, using Phospho-c-Fos (Ser32) (D82C12) XP ® Rabbit mAb (upper) and c-Fos (9F6) Rabbit mAb #2250 (lower). Antibody phospho-specificity is shown by treating lysates with λ-phosphatase.

c-Fos (9F6) Rabbit mAb - 2250 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 9F6
Western blot analysis of extracts from HeLa and H-4-II-E cells serum-starved overnight and TPA-stimulated for 4 hours, using c-Fos (9F6) Rabbit mAb.

c-Fos Antibody - 4384 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, RAW, and H-4-IIE cells serum-starved overnight and TPA-stimulated for 4 hours, using c-Fos Antibody.

c-Fos (4) - sc-52 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
c-Fos (4): sc-52. Nuclear immunofluorescence staining of methanol-fixed, phorbol ester-induced HeLa cells.

c-Fos (4-10G) - sc-413 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
c-Fos (4-10G): sc-413. Western blot analysis of human recombinant c-Fos immunopreciptated with c-Fos (4-10G): sc-413 and detected with c-Fos (K-25): sc-253.

c-Fos (6-2H-2F) - sc-447 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
c-Fos (6-2H-2F): sc-447. Western blot analysis of Fos gene family protein expression in A-431 (A) and Y79 (B) nuclear extracts.

c-Fos (C-10) - sc-271243 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
c-Fos (C-10): sc-271243. Western blot analysis of c-Fos expression in PMA treated Jurkat whole cell lysate.

c-Fos (E-8) - sc-166940 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
c-Fos (E-8): sc-166940. Western blot analysis of Fos B expression in non-transfected: sc-117752 (A) and human Fos B transfected: sc-177246 (B) 293T whole cell lysates.

c-Fos (H-125) - sc-7202 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
c-Fos (H-125): sc-7202. Western blot analysis of c-Fos expression in nuclear extracts prepared from untreated (A) and PMA-treated (B) Jurkat cells.

c-Fos (D-1) - sc-8047 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
c-Fos (D-1): sc-8047. Western blot analysis of c-Fos expression in NIH/3T3 nuclear extract (A) and from PMA-treated NIH/3T3 nuclear extract (B).

c-Fos (K-25) - sc-253 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
c-Fos (K-25) PE: sc-253 PE. Intracellular FCM analysis of fixed and permeabilized MIA PaCa-2a cells. Black line histogram represents the isotype control, normal rabbit IgG: sc-3871.

c-Fos (554C1a) - sc-81209 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
c-Fos (554C1a): sc-81209 Western Blot analysis of human recombinant c-Fos fusion protein.

p-c-Fos (34E4) - sc-81485 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
p-c-Fos (34E4): sc-81485. Western blot analysis of c-Fos phosphorylation in serum starved Hep G2 cells (A) and serum starved Hep G2 cells treated with 10 ng/ml EGF for 5 min (B), 15 min (C), 30 min (D), 1 hr (E) 2 hrs (F), 4 hrs (G) and 8 hrs (H).

p-c-Fos (Thr 232) - sc-130181 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-c-Fos (phospho S32) antibody - ab79319 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-c-Fos (phospho S32) antibody (ab79319) at 1/500 dilutionLane 1 : 24 hour starved Jurkat cell extractLane 2 : 24 hour starved Jurkat cell extract with Immunizing phosphopeptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-c-Fos (phospho S362) antibody - ab63444 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab63444 at 1/50 - 1/100 dilution staining c-Fos in human thyroid gland by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

Anti-c-Fos (phospho S362) antibody - ab192442 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-c-Fos (phospho S362) antibody (ab192442) at 1/500 dilutionLane 1 : Forskolin treated K562 whole cell lysate with antigen-specific peptideLane 2 : Forskolin treated K562 whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-c-Fos (phospho T232) antibody - ab17933 from Abcam

Prices $380.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-c-Fos (phospho T232) antibody - ab43175 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-c-Fos (phospho T232) antibody (ab43175) at 1 µg/mlLane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate - EGF treated Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : Hela Whole Cell Lysate - Bleomycin Treated (40U/ml)Lane 4 : Hela Whole Cell Lysate - Hydroxyurea Treated (48hr, 2uM) Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate - EGF treated with Human c-Fos (phospho T232) peptide (ab43652) at 1 µg/mlLane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human c-Fos (phospho T232) peptide (ab43652) at 1 µg/mlLane 7 : Hela Whole Cell Lysate - Bleomycin Treated (40U/ml) with Human c-Fos (phospho T232) peptide (ab43652) at 1 µg/mlLane 8 : Hela Whole Cell Lysate - Hydroxyurea Treated (48hr, 2uM) with Human c-Fos (phospho T232) peptide (ab43652) at 1 µg/mlLane 9 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate - EGF treated with Human c-Fos peptide (ab60297) at 1 µg/

Anti-c-Fos (phospho T232) antibody - ab193365 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling c-FOS with ab193365 at 1/50 dilution. Right panel was preincubated with blocking peptide.

Anti-c-Fos (phospho T232) antibody - ab138471 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-c-Fos (phospho T232) antibody (ab138471) at 1/500 dilution + Jurkat cell extract (from cells treated with EGF 200 ng/ml for 5 minutes) at 30 µg

Anti-c-Fos (phospho T325) antibody - ab27793 from Abcam

Prices $382.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-c-Fos (phospho T325) antibody (ab27793) at 1/1000 dilution (diluted in a 3% Milk TBST buffer.)Lane 1 : Non EGF treated A431 cell lysate Lane 2 : EGF treated A431 cell lysate Lane 3 : EGF treated A431 cell lysate treated with Lambda phosphataseSecondarygoat F(ab’)2 anti rabbit IgG HRP conjugate
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