Western blot analysis of extracts from untreated or serum-treated COS-7 cells exogenously expressing GFP-FoxO1, using Phospho-FoxO1 (Ser319) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after Western transfer.
Western blot analysis of extracts from serum-treated COS-7 cells untransfected, exogenously expressing FoxO4 or GFP-FoxO1, using Phospho-FoxO1 (Ser319) (left) or Phospho-FoxO1 (Ser319)/FoxO4 (Ser262) Antibody #2487 (right).