Western blot analysis of extracts from various cell lines using FoxP1 (D35D10) XP ® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using FoxP1 (D35D10) XP ® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Flow cytometric analysis of MOLT-4 cells using FoxP1 (D35D10) XP ® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 Raji cells and either 5 µl of FoxP1 (D35D10) XP ® Rabbit mAb, or 2 µl of Normal Rabbit IgG #2729, using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human BCL2 Promoter Primers #12924, SimpleChIP ® Human HSP90B1 Promoter Primers #12899, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.