Immunofluorescence of anti-CD9 Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Western blot of CD9 Antibody in HepG2 cell line lysates (35 ug/lane). CD9 (arrow) was detected using the purified antibody.
Western blot of CD9(arrow) using rabbit polyclonal CD9 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD9 gene (Lane 2) (Origene Technologies).
Western blot of lysate from HeLa cell line, using CD9 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot of lysate from mouse kidney tissue lysate, using CD9 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot of lysates from HeLa, MCF-7 cell line (from left to right), using CD9 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Western blot of lysates from HeLa, MCF-7 cell line (from left to right), using CD9 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Flow cytometric of Jurkat cells using CD9 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.