CD9

Gene Symbol CD9
Entrez Gene 928
Alt Symbol BTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29
Species Human
Gene Type protein-coding
Description CD9 molecule
Other Description 5H9 antigen|BA-2/p24 antigen|CD9 antigen|CD9 antigen (p24)|cell growth-inhibiting gene 2 protein|leukocyte antigen MIC3|motility related protein-1|tetraspanin-29
Swissprots Q5J7W6 P21926 Q96ES4 D3DUQ9
Accessions AAA51954 AAA51955 AAA51956 AAA51957 AAA51958 AAA51959 AAC60586 AAQ87878 CCF77003 EAW88812 EAW88813 P21926 AB079244 BAE71132 AI003581 AK296332 BAG59021 AK296894 BAG59453 AK298431 BAG60652 AK308700 AK309527 AK311020 AK315219 AY423720 AAS00483 AY966455 AAX78197 BC011988 AAH11988 BG291377 BM763066 DQ891314 ABM82240 DQ894498 ABM85424 HM245393 L34068 AAA59982 M38690 AAA80320 X60111 CAA42708 XM_005253814 XP_005253871 XM_011521040 XP_011519342 NM_001769 NP_001760
Function Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. {ECO:0000269|PubMed:14575715, ECO:0000269|PubMed:7511626, ECO:0000269|PubMed:8478605}.
Subcellular Location Membrane {ECO:0000269|PubMed:19640571}; Multi-pass membrane protein {ECO:0000269|PubMed:19640571}. Cell membrane {ECO:0000269|PubMed:19640571}; Multi-pass membrane protein {ECO:0000269|PubMed:19640571}.
Tissue Specificity Detected in platelets (at protein level). Expressed by a variety of hematopoietic and epithelial cells. {ECO:0000269|PubMed:19640571}.
Top Pathways Hematopoietic cell lineage

CD9 (D8O1A) Rabbit mAb - 13174 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D8O1A
Western blot analysis of extracts from various cell lines using CD9 (D8O1A) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

CD9 (D3H4P) Rabbit mAb - 13403 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3H4P
Western blot analysis of extracts from various cell lines using CD9 (D3H4P) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

CD9 (C-4) - sc-13118 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD9 (C-4): sc-13118. Western blot analysis of CD9 expression in BT-20 (A), ZR75-1 (B) and HeLa (C) whole cell lysates.

CD9 (H-110) - sc-9148 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CD9 (H-110): sc-9148. Western blot analysis of CD9 expression in human PBL whole cell lysate.

CD9 (N-19) - sc-7639 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

CD9 (P1/33/2) - sc-20048 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD9 siRNA (h): sc-35032. Immunofluorescence staining of methanol-fixed, control HeLa (A) and CD9 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with CD9 (P1/33/2): sc-20048.

CD9 (ALB 6) - sc-59140 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
CD9 (ALB 6): sc-59140. Western blot analysis of CD9 expression in human PBL whole cell lysate.

CD9 (MEM-61) - sc-51575 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
CD9 (MEM-61): sc-51575. Western blot analysis of CD9 expression in BT-20 (A) and ZR-75-1 (B) whole cell lysates under non-reducing conditions.

CD9 (SN4/C3-3A2) - sc-65251 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
CD9 (SN4/C3-3A2): sc-65251. Western blot analysis of CD9 expression in human PBL whole cell lysate.

Anti-CD9 antibody - ab97999 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of CD9 staining in Human Lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97999, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CD9 antibody - ab189831 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-CD9 antibody (ab189831) at 1/1000 dilutionLane 1 : Mouse brain total cell extractsLane 2 : Mouse thymus total cell extracts

Anti-CD9 antibody - ab27542 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Ab27542, at a dilution of 1/50, staining CD9 in formalin fixed paraffin embedded human tonsil tissue section with by Immunohistochemistry.

Anti-CD9 antibody - ab155825 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-CD9 antibody (ab155825) at 1/1000 dilution + K562 whole cell lysate at 30 µg

Anti-CD9 antibody [5G6] - ab124476 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5G6
All lanes : Anti-CD9 antibody [5G6] (ab124476) at 1/500 dilutionLane 1 : HEK293 cell lysate, non-transfected Lane 2 : HEK293 cell lysate, transfected with CD9 (aa 37-228)-hIgGFc

Anti-CD9 antibody [EPR2949] - ab92726 from Abcam

Prices $403.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2949
All lanes : Anti-CD9 antibody [EPR2949] (ab92726) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HuT-78 cell lysateLane 3 : U87-MG cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit IgG at 1/2000 dilution

Anti-CD9 antibody [EPR2949] (Alexa Fluor® 488) - ab195348 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2949
ab195348 staining CD9 in MCF7 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195348 at a working dilution of 1 in 50 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-CD9 antibody [EPR2949] (Alexa Fluor® 647) - ab195349 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2949
ab195349 staining CD9 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195349 at 1/50 dilution(shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-CD9 antibody [EPR2949] (HRP) - ab195552 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2949
IHC image of CD9 staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195552 at 1/71 dilution for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Anti-CD9 antibody [MEM-61] - ab2215 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MEM-61
IHC image of ab2215 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2215, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CD9 antibody [MEM-61] (Alexa Fluor® 647) - ab187776 from Abcam

Prices $370.00
Sizes 100 tests
Host Mouse
Clonality Monoclonal
Clone MEM-61

Anti-CD9 antibody [MEM-61] (Biotin) - ab28094 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MEM-61

Anti-CD9 antibody [MEM-61] (FITC) - ab18241 from Abcam

Prices $186.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone MEM-61

Anti-CD9 antibody [MEM-61], prediluted (Allophycocyanin) - ab82389 from Abcam

Prices $370.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone MEM-61

Anti-CD9 antibody [MM2/57] - ab61873 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MM2/57

Anti-CD9 antibody, prediluted - ab27566 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
Ab27566, prediluted, staining CD9 in formalin fixed, paraffin embedded human tonsil tissue sections by Immunohistochemistry.
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