Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
The anti-Phospho-CHK1-S317 antibody is used in Western blot for detection in, from left to right, Jurkat, A2058, HeLa, and HL60 tissue lysates.
Dot blot of anti-Phospho-CHK1-S317 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibodies working concentration was 0.5ug per ml.