Western Blot analysis of CRX expression in transfected 293T cell line by CRX monoclonal antibody (M01), clone F6-C2.Lane 1: CRX transfected lysate(32.261 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged CRX is approximately 1 ng/ml as a capture antibody.
Western blot analysis of CRX over-expressed 293 cell line, cotransfected with CRX Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CRX monoclonal antibody (M01), clone F6-C2 . GAPDH ( 36.1 kDa ) used as specificity and loading control.