Western blot analysis of extracts from various cell lines using Glut1 (D3J3A) Rabbit mAb.
Western blot analysis of extracts from Hep G2 cells transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® Glut1 siRNA I (+) #14184, using Glut1 (D3J3A) Rabbit mAb (upper) or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower).
Immunoprecipitation of Glut1 from Huh6 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Glut1 (D3J3A) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Glut1 (D3J3A) Rabbit mAb. Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 was used as the secondary antibody.