Western blot analysis of extracts from serum starved NIH/3T3 cells, untreated or treated with insulin (100 nM, 5 min), hIGF-1 #8917 (100 ng/ml, 5 min), or λ phosphatase, using Phospho-GRB10 (Ser476) (D4E6) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of phospho-GRB10 (Ser476) from extracts of serum starved NIH/3T3 cells treated with insulin (100 nM, 5 min), using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Phospho-GRB10 (Ser476) (D4E6) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-GRB10 (Ser476) (D4E6) Rabbit mAb.