Western blot analysis of extracts from COS-7 cells, λ phosphatase- or PDGF-treated (100 μg/ml, 15 min), and PC-3 cells, untreated or λ phosphatase-treated, using Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb (upper) and GSK-3α/β (D75D3) XP ® Rabbit mAb #5676 (lower).
Western blot analysis of extracts from GSK-3α (-/-) and GSK-3β (-/-) mouse embryonic fibroblasts (MEF), untreated or insulin-treated (100 ng/ml, 20 min) , using Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb (upper) and GSK-3α/β (D75D3) XP ® Rabbit mAb #5676 (lower). (MEF GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).