Formalin-fixed and paraffin-embedded human breast carcinoma with CTSK Antibody (Center R222), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of CTSK (arrow) using rabbit polyclonal CTSK Antibody (Center R222). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CTSK gene (Lane 2).
Flow cytometric of ZR-75-1 cells using CTSK Antibody (Center R222)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.