Western blot analysis of extracts from HeLa cells, mock transfected or transfected with SignalSilence® GSK-3α/β siRNA #6301, using GSK-3α/β (D75D3) XP ® Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).
Western blot analysis of extracts from GSK-3β (-/-) (lanes 1,2), GSK-3α (-/-) (lanes 3,4) and wild type (lanes 5,6) mouse embryonic fibroblast cells (MEF), untreated or insulin treated, using GSK-3α/β (D75D3) XP ® Rabbit mAb. (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).