Confocal immunofluorescent analysis of MEF/GSK-3 wildtype cells (left), MEF/GSK-3β (-/-) cells (center) and MEF/GSK-3α (-/-) cells (right), using GSK-3α (D80D1) XP ® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye). (MEF/wildtype, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).
Flow cytometric analysis of MEF/GSK-3α (-/-) cells (red), MEF/GSK-3β (-/-) cells (green), and MEF/GSK-3 wildtype cells (blue) using GSK-3α (D80D1) XP ® Rabbit mAb. (MEF/GSK-3 wildtype, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).