ImmunofluorescenceNRK cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 5 μg/mL Rabbit Anti-WASH1 (C-terminal) (
Cat. No. SAB4200373). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (
Cat. No. 18772).
ImmunoblottingCell line lysates were separated on SDS-PAGE and probed with 5 μg/mL Rabbit Anti-WASH1 (C-terminal) (
Cat. No. SAB4200373). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. HEK-293T overexpressing human WASH1
2. K562
3. KATO III
4. HeLa
5. NRK
6. PMC2
7. A20
8. ACHN
9. RAW264