WASH1

Gene Symbol WASH1
Entrez Gene 100287171
Alt Symbol FAM39E, WASH
Species Human
Gene Type protein-coding
Description WAS protein family homolog 1
Other Description CXYorf1-like protein on chromosome 9|FLJ00075 protein|family with sequence similarity 39, member E
Swissprots A8K0Z3
Accessions A8K0Z3 ABX71510 AK024448 BAB15738 AK024481 BAB15771 AK289708 BAF82397 AK309568 BC014406 BC063893 BC094860 BC156859 AAI56860 BC160141 AAI60141 BM970740 CN404407 XM_011517658 XP_011515960 XM_011517659 XP_011515961 XM_011517660 XP_011515962 XM_011517661 XP_011515963 XM_011517662 XP_011515964 XM_011517663 XP_011515965 XM_011517664 XP_011515966 XM_011517665 XP_011515967 XM_011517666 XP_011515968 XM_011517667 XP_011515969 XM_011517668 XP_011515970 XM_011517669 XP_011515971 XM_011517670 XP_011515972 XM_011517671 XP_011515973 XM_011517672 XP_011515974 XR_929142 XR_929143 NM_182905 NP_878908
Function Acts as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922874, PubMed:19922875, PubMed:20498093, PubMed:23452853). Its assembly in the WASH core complex seems to inhibit its NPF activity and via FAM21 is required for its membrane targeting (PubMed:20498093). Involved in endocytic trafficking of EGF (By similarity). Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration (PubMed:22114305). In T cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility
Subcellular Location Early endosome membrane {ECO:0000269|PubMed:19922874}. Recycling endosome membrane {ECO:0000250|UniProtKB:Q8VDD8}. Late endosome {ECO:0000269|PubMed:22114305, ECO:0000269|PubMed:24344185}. Cytoplasmic vesicle, autophagosome {ECO:0000250|UniProtKB:Q8VDD8}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250|UniProtKB:Q8VDD8}. Note=Localization to the endosome membrane is mediated via its interaction with FAM21 (PubMed:19922874). Localizes to MMP14- positive late endosomes and transiently to invadipodia (PubMed:24344185). Localized to Salmonella typhimurium entry sites (By similarity). {ECO:0000250|UniProtKB:Q8VDD8, ECO:0000269|PubMed:19922874, ECO:0000269|PubMed:24344185}.

Monoclonal Anti-WASH1 antibody produced in mouse - SAB4200552 from Sigma-Aldrich

Prices $337.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone WASH1-27
<B>Immunofluorescence</B><BR/>WT MEF cells (WASH +/+) or WASHout MEF cells (WASH-/-) were fixed in 4% PFA for 20 minutes and permeabilized with 0.15% Surfactamps for 3 minutes. Fixed cells were stained with 1:300 dilution of Monoclonal Anti-WASH1 Clone: WASH1-27 (<B>Cat. No. SAB4200552</B>). The antibody was developed using Goat Anti-Mouse IgG, TRITC conjugate. Cells were counterstained with Hoechst (blue) to stain nuclei. Pictures are courtesy of Timothy S. Gomez and Daniel D. Billadeau, Departments of Biochemistry and Molecular Biology and Immunology, Division of Oncology Research, Mayo Clinic, Rochester, USA.

Anti-WASH1 antibody produced in rabbit - SAB4200372 from Sigma-Aldrich

Prices $343.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Whole extract of HEK-293T cells overexpressing mouse WASH1 was separated on SDS-PAGE and probed with Rabbit Anti-WASH1 (<B>Cat. No. SAB4200372</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/>Lanes<BR/>1. 2 μg/mL antibody<BR/>2. 4 μg/mL antibody

Purified anti-WASH1 Antibody - 657702 from Biolegend

Prices $205.00
Sizes 100 µg
Host Mouse
Clone P1B1-B1
Hela cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-WASH-1 antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.

Anti-WASH1 (C-terminal) polyclonal antibody - DPABH-05914 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

Anti-WAS protein family homolog 1 (NT) Antibody - ABS73 from EMD Millipore

Prices $269.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-WAS protein family homolog 1 (CT) Antibody - ABS72 from EMD Millipore

Host Rabbit
Clonality Polyclonal
Western Blotting Analysis: <br />Representative lot data. <br />HeLa cell lysate was probed with Anti-WAS protein family homolog 1 (C-terminus) (1 µg/mL). <br />Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and chemiluminescence detection system. <br />Arrow indicates WAS protein family homolog 1 (~60 kDa).

Anti-WASH1 (C-terminal) antibody produced in rabbit - SAB4200373 from Sigma-Aldrich

Prices $354.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>NRK cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 5 μg/mL Rabbit Anti-WASH1 (C-terminal) (<B>Cat. No. SAB4200373</B>). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (<B>Cat. No. 18772</B>).

WASH1 antibody - TA352339 from Acris Antibodies

Prices $325.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone P1B1-B1
Hela cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-WASH-1 antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
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